› 2xwa

X-ray diffraction
2.8Å resolution

Crystal Structure of Complement Factor D Mutant R202A

Released:
DOI: 10.2210/pdb2xwa/pdb
PDB entry 2xwa coloured by chain, front view.
PDB entry 2xwa coloured by chain, side view.
PDB entry 2xwa coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Structures of C3b in complex with factors B and D give insight into complement convertase formation.
Forneris F, Ricklin D, Wu J, Tzekou A, Wallace RS, Lambris JD, Gros P
Science 330 1816-20 (2010)
PMID: 21205667

Function and Biology Details

Reaction catalysed: 
Selective cleavage of Arg-|-Lys bond in complement factor B when in complex with complement subcomponent C3b or with cobra venom factor.
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-133224 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Complement factor D Chains: A, B
Molecule details ›
Chains: A, B
Length: 228 amino acids
Theoretical weight: 24.35 KDa
Source organism: Homo sapiens
Expression system: Homo sapiens
UniProt:
  • Canonical: P00746 (Residues: 26-253; Coverage: 98%)
Gene names: CFD, DF, PFD
Sequence domains: Trypsin
Structure domains: Trypsin-like serine proteases

Ligands and Environments

1 bound ligand:
Ligand GOL 2 x GOL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SLS BEAMLINE X06SA
Spacegroup: P212121
Unit cell:
a: 44.222Å b: 67.491Å c: 133.588Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.24 0.238 0.283
Expression system: Homo sapiens

Quick links

Citations

49 review citations

Complement System Part I - Molecular Mechanisms of Activation and Regulation.
Merle et al. (2015)
48 more

2 mentions without citation

Allostery in trypsin-like proteases suggests new therapeutic strategies.
Gohara et al. (2011)
1 more