› 2xxb

X-ray diffraction
2.15Å resolution

Penta-mutant of Thermus thermophilus lactate dehydrogenase, complex with AMP

Released:
DOI: 10.2210/pdb2xxb/pdb
PDB entry 2xxb coloured by chain, front view.
PDB entry 2xxb coloured by chain, side view.
PDB entry 2xxb coloured by chain, top view.
Source organism: Thermus thermophilus HB8
Entry authors: Diop F, Coquelle N, Vellieux FMD

Function and Biology Details

Reaction catalysed: 
(S)-lactate + NAD(+) = pyruvate + NADH
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-178009 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
L-lactate dehydrogenase Chains: A, B
Molecule details ›
Chains: A, B
Length: 310 amino acids
Theoretical weight: 32.6 KDa
Source organism: Thermus thermophilus HB8
Expression system: Escherichia coli DH5[alpha]
UniProt:
  • Canonical: Q5SJA1 (Residues: 1-310; Coverage: 100%)
Gene names: TTHA1113, ldh
Sequence domains:
Structure domains:

Ligands and Environments

1 bound ligand:
Ligand AMP 2 x AMP
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID14-1
Spacegroup: C2221
Unit cell:
a: 54.99Å b: 153.47Å c: 150.61Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.223 0.219 0.289
Expression system: Escherichia coli DH5[alpha]

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