› 2xzr

X-ray diffraction
2.8Å resolution

Escherichia coli Immunoglobulin-binding protein EibD 391-438 FUSED TO GCN4 ADAPTORS

Released:
DOI: 10.2210/pdb2xzr/pdb
PDB entry 2xzr coloured by chain, front view.
PDB entry 2xzr coloured by chain, side view.
PDB entry 2xzr coloured by chain, top view.
Source organism: Enterobacteria phage P-EibD
Primary publication:
The structure of E. coli IgG-binding protein D suggests a general model for bending and binding in trimeric autotransporter adhesins.
Leo JC, Lyskowski A, Hattula K, Hartmann MD, Schwarz H, Butcher SJ, Linke D, Lupas AN, Goldman A
Structure 19 1021-30 (2011)
PMID: 21742268

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domain:

Structure analysis Details

Assembly composition:
homo trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-192395 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Immunoglobulin-binding protein EibD Chain: A
Molecule details ›
Chain: A
Length: 114 amino acids
Theoretical weight: 13.55 KDa
Source organism: Enterobacteria phage P-EibD
Expression system: Escherichia coli
UniProt:
  • Canonical: Q9MCI8 (Residues: 385-441; Coverage: 12%)
Gene name: eibD
Structure domains: Single alpha-helices involved in coiled-coils or other helix-helix interfaces

Ligands and Environments

1 bound ligand:
Ligand CL 4 x CL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SLS BEAMLINE X06DA
Spacegroup: P321
Unit cell:
a: 36.67Å b: 36.67Å c: 228.58Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.265 0.26 0.308
Expression system: Escherichia coli

Quick links

Citations

14 review citations

Type V secretion: mechanism(s) of autotransport through the bacterial outer membrane.
Leo et al. (2012)
13 more

1 mention without citation

Exploring the atomic structure and conformational flexibility of a 320 Å long engineered viral fiber using X-ray crystallography.
Bhardwaj et al. (2014)