› 2xzv

X-ray diffraction
1.6Å resolution

The cyanobacterial PP2C-like phosphatase tPphA requires three metals in the catalytic center for efficient catalysis

Released:
DOI: 10.2210/pdb2xzv/pdb
PDB entry 2xzv coloured by chain, front view.
PDB entry 2xzv coloured by chain, side view.
PDB entry 2xzv coloured by chain, top view.
Source organism: Synechococcus elongatus
Primary publication:
A third metal is required for catalytic activity of the signal-transducing protein phosphatase M tPphA.
Su J, Schlicker C, Forchhammer K
J Biol Chem 286 13481-8 (2011)
PMID: 21310952

Function and Biology Details

Reaction catalysed: 
[a protein]-serine/threonine phosphate + H(2)O = [a protein]-serine/threonine + phosphate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-184051 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
protein-serine/threonine phosphatase Chain: A
Molecule details ›
Chain: A
Length: 240 amino acids
Theoretical weight: 26.69 KDa
Source organism: Synechococcus elongatus
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q8DGS1 (Residues: 1-240; Coverage: 100%)
Gene name: tlr2243
Sequence domains: Protein phosphatase 2C
Structure domains: PPM-type phosphatase domain

Ligands and Environments

2 bound ligands:
Ligand CA 2 x CA
Ligand MG 2 x MG
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE BM14
Spacegroup: C2221
Unit cell:
a: 38.646Å b: 151.436Å c: 82.603Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.212 0.21 0.242
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

4 review citations

Protein Serine/Threonine Phosphatases: Keys to Unlocking Regulators and Substrates.
Brautigan et al. (2018)
3 more

2 mentions without citation

A third metal is required for catalytic activity of the signal-transducing protein phosphatase M tPphA.
Su et al. (2011)
1 more