› 2y0f

X-ray diffraction
2.5Å resolution

STRUCTURE OF GCPE (IspG) FROM THERMUS THERMOPHILUS HB27

Released:
DOI: 10.2210/pdb2y0f/pdb
PDB entry 2y0f coloured by chain, front view.
PDB entry 2y0f coloured by chain, side view.
PDB entry 2y0f coloured by chain, top view.
Source organism: Thermus thermophilus HB27
Primary publication:
Structure of the E-1-hydroxy-2-methyl-but-2-enyl-4-diphosphate synthase (GcpE) from Thermus thermophilus.
Rekittke I, Nonaka T, Wiesner J, Demmer U, Warkentin E, Jomaa H, Ermler U
FEBS Lett 585 447-51 (2011)
PMID: 21167158

Function and Biology Details

Reaction catalysed: 
(E)-4-hydroxy-3-methylbut-2-en-1-yl diphosphate + H(2)O + oxidized flavodoxin = 2-C-methyl-D-erythritol 2,4-cyclodiphosphate + reduced flavodoxin
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-180818 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
4-hydroxy-3-methylbut-2-en-1-yl diphosphate synthase (flavodoxin) Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 406 amino acids
Theoretical weight: 44.28 KDa
Source organism: Thermus thermophilus HB27
Expression system: Escherichia coli
UniProt:
  • Canonical: Q72H18 (Residues: 1-406; Coverage: 100%)
Gene names: TT_C1677, gcpE, ispG
Sequence domains: GcpE protein
Structure domains:

Ligands and Environments

1 bound ligand:
Ligand SF4 4 x SF4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SLS BEAMLINE X10SA
Spacegroup: P21
Unit cell:
a: 84.5Å b: 130.97Å c: 101.26Å
α: 90° β: 102.1° γ: 90°
R-values:
R R work R free
0.202 0.199 0.263
Expression system: Escherichia coli

Quick links

Citations

6 review citations

Terpene biosynthesis: modularity rules.
Oldfield et al. (2012)
5 more

4 mentions without citation

Bioorganometallic chemistry with IspG and IspH: structure, function, and inhibition of the [Fe(4)S(4)] proteins involved in isoprenoid biosynthesis.
Wang et al. (2014)
3 more