› 2y22

X-ray diffraction
3.7Å resolution

Human AlphaB-crystallin Domain (residues 67-157)

Released:
DOI: 10.2210/pdb2y22/pdb
PDB entry 2y22 coloured by chain, front view.
PDB entry 2y22 coloured by chain, side view.
PDB entry 2y22 coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Crystal structure of R120G disease mutant of human αB-crystallin domain dimer shows closure of a groove.
Clark AR, Naylor CE, Bagnéris C, Keep NH, Slingsby C
J Mol Biol 408 118-34 (2011)
PMID: 21329698

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-135873 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Alpha-crystallin B chain Chains: A, B, C, D, E, F
Molecule details ›
Chains: A, B, C, D, E, F
Length: 94 amino acids
Theoretical weight: 10.89 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P02511 (Residues: 67-157; Coverage: 52%)
Gene names: CRYA2, CRYAB, HSPB5
Sequence domains: Hsp20/alpha crystallin family
Structure domains: Immunoglobulin-like

Ligands and Environments

No bound ligands
1 modified residue:
Ligand MSE 18 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID14-4
Spacegroup: P21212
Unit cell:
a: 67.28Å b: 78.34Å c: 131.4Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.216 0.213 0.277
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

20 review citations

A first line of stress defense: small heat shock proteins and their function in protein homeostasis.
Haslbeck et al. (2015)
19 more

3 mentions without citation

Mechanisms of Small Heat Shock Proteins.
Janowska et al. (2019)
2 more