› 2y6z

X-ray diffraction
2.6Å resolution

Crystallographic structure of GM23 an example of Catalytic migration from TIM to thiamin phosphate synthase.

Released:
DOI: 10.2210/pdb2y6z/pdb
PDB entry 2y6z coloured by chain, front view.
PDB entry 2y6z coloured by chain, side view.
PDB entry 2y6z coloured by chain, top view.
Source organism: Trypanosoma brucei brucei
Primary publication:
Evolutionary walk between (β/α)(8) barrels: catalytic migration from triosephosphate isomerase to thiamin phosphate synthase.
Saab-Rincón G, Olvera L, Olvera M, Rudiño-Piñera E, Benites E, Soberón X, Morett E
J Mol Biol 416 255-70 (2012)
PMID: 22226942

Function and Biology Details

Reaction catalysed: 
D-glyceraldehyde 3-phosphate = glycerone phosphate
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-138176 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Triosephosphate isomerase, glycosomal Chain: A
Molecule details ›
Chain: A
Length: 256 amino acids
Theoretical weight: 27.16 KDa
Source organism: Trypanosoma brucei brucei
Expression system: Escherichia coli
UniProt:
  • Canonical: P04789 (Residues: 1-47, 49-250; Coverage: 97%)
Sequence domains: Triosephosphate isomerase
Structure domains: Aldolase class I

Ligands and Environments

2 bound ligands:
Ligand TPS 1 x TPS
Ligand POP 1 x POP
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X6A
Spacegroup: P6522
Unit cell:
a: 126.3Å b: 126.3Å c: 107.29Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.191 0.194 0.235
Expression system: Escherichia coli

Quick links

Citations

2 review citations

A guide to the effects of a large portion of the residues of triosephosphate isomerase on catalysis, stability, druggability, and human disease.
Olivares-Illana et al. (2017)
1 more

1 mention without citation

The Potential of Secondary Metabolites from Plants as Drugs or Leads against Protozoan Neglected Diseases-Part III: In-Silico Molecular Docking Investigations.
Ogungbe et al. (2016)