› 2y7s

X-ray diffraction
1.9Å resolution

Structure of a designed meningococcal antigen (factor H binding protein, mutant G1) inducing broad protective immunity

Released:
DOI: 10.2210/pdb2y7s/pdb
PDB entry 2y7s coloured by chain, front view.
PDB entry 2y7s coloured by chain, side view.
PDB entry 2y7s coloured by chain, top view.
Source organism: Neisseria meningitidis MC58
Primary publication:
Rational design of a meningococcal antigen inducing broad protective immunity.
Scarselli M, Aricò B, Brunelli B, Savino S, Di Marcello F, Palumbo E, Veggi D, Ciucchi L, Cartocci E, Bottomley MJ, Malito E, Lo Surdo P, Comanducci M, Giuliani MM, Cantini F, Dragonetti S, Colaprico A, Doro F, Giannetti P, Pallaoro M, Brogioni B, Tontini M, Hilleringmann M, Nardi-Dei V, Banci L, Pizza M, Rappuoli R
Sci Transl Med 3 91ra62 (2011)
PMID: 21753121

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-191680 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Factor H binding protein Chains: A, B
Molecule details ›
Chains: A, B
Length: 256 amino acids
Theoretical weight: 27.72 KDa
Source organism: Neisseria meningitidis MC58
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q9JXV4 (Residues: 27-274; Coverage: 97%)
Gene names: NMB1870, fhbP
Sequence domains:
Structure domains:

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 5.0.3
Spacegroup: P21
Unit cell:
a: 47.48Å b: 99.65Å c: 58.06Å
α: 90° β: 97.82° γ: 90°
R-values:
R R work R free
0.188 0.186 0.233
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

39 review citations

From empiricism to rational design: a personal perspective of the evolution of vaccine development.
De Gregorio et al. (2014)
38 more

5 mentions without citation

The new multicomponent vaccine against meningococcal serogroup B, 4CMenB: immunological, functional and structural characterization of the antigens.
Serruto et al. (2012)
4 more