› 2ya9

X-ray diffraction
2.3Å resolution

Crystal structure of the autoinhibited form of mouse DAPK2

Released:
DOI: 10.2210/pdb2ya9/pdb
PDB entry 2ya9 coloured by chain, front view.
PDB entry 2ya9 coloured by chain, side view.
PDB entry 2ya9 coloured by chain, top view.
Source organism: Mus musculus
Primary publication:
Structure of the dimeric autoinhibited conformation of DAPK2, a pro-apoptotic protein kinase.
Patel AK, Yadav RP, Majava V, Kursula I, Kursula P
J Mol Biol 409 369-83 (2011)
PMID: 21497605

Function and Biology Details

Reaction catalysed: 
ATP + a protein = ADP + a phosphoprotein
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-186562 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Death-associated protein kinase 2 Chains: A, B
Molecule details ›
Chains: A, B
Length: 361 amino acids
Theoretical weight: 41.8 KDa
Source organism: Mus musculus
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q8VDF3 (Residues: 11-370; Coverage: 97%)
Gene name: Dapk2
Sequence domains: Protein kinase domain
Structure domains:

Ligands and Environments

2 bound ligands:
Ligand D1D 2 x D1D
Ligand CA 1 x CA
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID14-2
Spacegroup: P212121
Unit cell:
a: 64.04Å b: 86.44Å c: 124.27Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.209 0.206 0.261
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

7 review citations

The DAPK family: a structure-function analysis.
Shiloh et al. (2014)
6 more

1 mention without citation

Disordered Protein Kinase Regions in Regulation of Kinase Domain Cores.
Gógl et al. (2019)