› 2yeq

X-ray diffraction
1.93Å resolution

Function and Biology Details

Reaction catalysed: 
A phosphate monoester + H(2)O = an alcohol + phosphate
Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assemblies composition:
monomeric (preferred)
homo dimer
Assembly name:
PDBe Complex ID:
PDB-CPX-154579 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Alkaline phosphatase D Chains: A, B
Molecule details ›
Chains: A, B
Length: 527 amino acids
Theoretical weight: 59.79 KDa
Source organism: Bacillus subtilis
Expression system: Escherichia coli
UniProt:
  • Canonical: P42251 (Residues: 57-583; Coverage: 100%)
Gene names: BSU02620, phoD, ycbS
Sequence domains:
Structure domains:

Ligands and Environments

6 bound ligands:
Ligand PE5 30 x PE5
Ligand EDO 43 x EDO
Ligand NA 6 x NA
Ligand FE 2 x FE
Ligand CA 4 x CA
Ligand PO4 2 x PO4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: DIAMOND BEAMLINE I03
Spacegroup: I4122
Unit cell:
a: 147.318Å b: 147.318Å c: 347.646Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.18 0.178 0.202
Expression system: Escherichia coli

Quick links

Citations

1 review citation

Microbial Surface Colonization and Biofilm Development in Marine Environments.
Dang et al. (2016)
24 other articles

5 mentions without citation

Crystal structure of the Bacillus subtilis phosphodiesterase PhoD reveals an iron and calcium-containing active site.
Rodriguez et al. (2014)
4 more