› 2yhs

X-ray diffraction
1.6Å resolution

Structure of the E. coli SRP receptor FtsY

Released:
DOI: 10.2210/pdb2yhs/pdb
PDB entry 2yhs coloured by chain, front view.
PDB entry 2yhs coloured by chain, side view.
PDB entry 2yhs coloured by chain, top view.
Source organism: Escherichia coli K-12
Primary publication:
Lipids trigger a conformational switch that regulates signal recognition particle (SRP)-mediated protein targeting.
Stjepanovic G, Kapp K, Bange G, Graf C, Parlitz R, Wild K, Mayer MP, Sinning I
J Biol Chem 286 23489-97 (2011)
PMID: 21543314

Function and Biology Details

Reaction catalysed: 
GTP + H(2)O = GDP + phosphate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-145174 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Signal recognition particle receptor FtsY Chain: A
Molecule details ›
Chain: A
Length: 503 amino acids
Theoretical weight: 55.4 KDa
Source organism: Escherichia coli K-12
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P10121 (Residues: 1-497; Coverage: 100%)
Gene names: JW3429, b3464, ftsY
Sequence domains:
Structure domains:

Ligands and Environments

1 bound ligand:
Ligand EDO 5 x EDO
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID14-1
Spacegroup: P21212
Unit cell:
a: 79.358Å b: 108.412Å c: 31.902Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.18 0.178 0.22
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

6 review citations

Signal recognition particle: an essential protein-targeting machine.
Akopian et al. (2013)
5 more

6 mentions without citation

The GTPase activity of FlhF is dispensable for flagellar localization, but not motility, in Pseudomonas aeruginosa.
Schniederberend et al. (2013)
5 more