› 2z00

X-ray diffraction
2.42Å resolution

Crystal structure of dihydroorotase from Thermus thermophilus

Released:
DOI: 10.2210/pdb2z00/pdb
PDB entry 2z00 coloured by chain, front view.
PDB entry 2z00 coloured by chain, side view.
PDB entry 2z00 coloured by chain, top view.
Source organism: Thermus thermophilus HB8
Entry authors: Kanagawa M, Baba S, Kuramitsu S, Yokoyama S, Kawai G, Sampei G, RIKEN Structural Genomics/Proteomics Initiative (RSGI)

Function and Biology Details

Reaction catalysed: 
(S)-dihydroorotate + H(2)O = N-carbamoyl-L-aspartate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assemblies composition:
monomeric (preferred)
homo dimer
Assembly name:
PDBe Complex ID:
PDB-CPX-178068 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Dihydroorotase Chain: A
Molecule details ›
Chain: A
Length: 426 amino acids
Theoretical weight: 45.94 KDa
Source organism: Thermus thermophilus HB8
Expression system: Escherichia coli
UniProt:
  • Canonical: Q5SK67 (Residues: 1-426; Coverage: 100%)
Gene names: TTHA0781, pyrC
Sequence domains: Amidohydrolase family
Structure domains:

Ligands and Environments

1 bound ligand:
Ligand ZN 2 x ZN
1 modified residue:
Ligand MSE 4 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: SPRING-8 BEAMLINE BL26B2
Spacegroup: P6522
Unit cell:
a: 129.937Å b: 129.937Å c: 209.906Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.188 0.188 0.192
Expression system: Escherichia coli

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