› 2zab

X-ray diffraction
1.66Å resolution

Crystal Structure of Family 7 Alginate Lyase A1-II' Y284F in Cmplex with Product (GGG)

Released:
DOI: 10.2210/pdb2zab/pdb
PDB entry 2zab coloured by chain, front view.
PDB entry 2zab coloured by chain, side view.
PDB entry 2zab coloured by chain, top view.
Source organism: Sphingomonas sp. A1
Entry authors: Ogura K, Yamasaki M, Mikami B, Hashimoto W, Murata K

Function and Biology Details

Reaction catalysed: 
Eliminative cleavage of alginate to give oligosaccharides with 4-deoxy-alpha-L-erythro-hex-4-enuronosyl groups at their non-reducing ends and beta-D-mannuronate at their reducing end.
Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-181130 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecules (2 distinct):
Alginate lyase 2 domain-containing protein Chain: A
Molecule details ›
Chain: A
Length: 236 amino acids
Theoretical weight: 25.78 KDa
Source organism: Sphingomonas sp. A1
Expression system: Escherichia coli
UniProt:
  • Canonical: Q75WP3 (Residues: 81-308; Coverage: 74%)
Gene name: aly
Sequence domains: Alginate lyase
Structure domains: Jelly Rolls

Ligands and Environments

Carbohydrate polymer : NEW Components: LGU, BEM
Carbohydrate polymer
1 bound ligand:
Ligand GOL 1 x GOL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SPRING-8 BEAMLINE BL38B1
Spacegroup: P212121
Unit cell:
a: 34.73Å b: 68.102Å c: 79.95Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.162 0.16 0.194
Expression system: Escherichia coli

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