› 2zbg

X-ray diffraction
2.55Å resolution

Calcium pump crystal structure with bound AlF4 and TG in the absence of calcium

Released:
DOI: 10.2210/pdb2zbg/pdb
PDB entry 2zbg coloured by chain, front view.
PDB entry 2zbg coloured by chain, side view.
PDB entry 2zbg coloured by chain, top view.
Source organism: Oryctolagus cuniculus
Primary publication:
How processing of aspartylphosphate is coupled to lumenal gating of the ion pathway in the calcium pump.
Toyoshima C, Norimatsu Y, Iwasawa S, Tsuda T, Ogawa H
Proc Natl Acad Sci U S A 104 19831-6 (2007)
PMID: 18077416

Function and Biology Details

Reaction catalysed: 
ATP + H(2)O + Ca(2+)(Side 1) = ADP + phosphate + Ca(2+)(Side 2)
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-137501 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:

Ligands and Environments

3 bound ligands:
Ligand MG 1 x MG
Ligand ALF 1 x ALF
Ligand TG1 1 x TG1
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SPRING-8 BEAMLINE BL41XU
Spacegroup: C2
Unit cell:
a: 177.5Å b: 70.2Å c: 143.4Å
α: 90° β: 106.8° γ: 90°
R-values:
R R work R free
0.224 0.224 0.248

Quick links

Citations

17 review citations

Ion channels versus ion pumps: the principal difference, in principle.
Gadsby DC. (2009)
16 more

7 mentions without citation

Improved low-resolution crystallographic refinement with Phenix and Rosetta.
DiMaio et al. (2013)
6 more