› 2zca

X-ray diffraction
1.8Å resolution

Crystal Structure of TTHB189, a CRISPR-associated protein, Cse2 family from Thermus thermophilus HB8

Released:
DOI: 10.2210/pdb2zca/pdb
PDB entry 2zca coloured by chain, front view.
PDB entry 2zca coloured by chain, side view.
PDB entry 2zca coloured by chain, top view.
Source organism: Thermus thermophilus
Primary publication:
X-ray crystal structure of a CRISPR-associated protein, Cse2, from Thermus thermophilus HB8.
Agari Y, Yokoyama S, Kuramitsu S, Shinkai A
Proteins 73 1063-7 (2008)
PMID: 18798563

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-176132 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
CRISPR-associated protein Cse2 Chains: A, B
Molecule details ›
Chains: A, B
Length: 169 amino acids
Theoretical weight: 19.53 KDa
Source organism: Thermus thermophilus
Expression system: Escherichia coli
UniProt:
  • Canonical: Q53VY0 (Residues: 1-169; Coverage: 100%)
Gene names: TTHB189, cse2
Sequence domains: CRISPR-associated protein Cse2 (CRISPR_cse2)
Structure domains: CRISPR-associated protein Cse2

Ligands and Environments

No bound ligands
1 modified residue:
Ligand MSE 4 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: SPRING-8 BEAMLINE BL26B1
Spacegroup: P21
Unit cell:
a: 52.081Å b: 71.238Å c: 53.753Å
α: 90° β: 115.78° γ: 90°
R-values:
R R work R free
0.189 0.186 0.215
Expression system: Escherichia coli

Quick links

Citations

6 review citations

CRISPR-based adaptive and heritable immunity in prokaryotes.
van der Oost et al. (2009)
5 more

7 mentions without citation

Evolution and classification of the CRISPR-Cas systems.
Makarova et al. (2011)
6 more