› 2zej

X-ray diffraction
2Å resolution

Structure of the ROC domain from the Parkinson's disease-associated leucine-rich repeat kinase 2 reveals a dimeric GTPase

Released:
DOI: 10.2210/pdb2zej/pdb
PDB entry 2zej coloured by chain, front view.
PDB entry 2zej coloured by chain, side view.
PDB entry 2zej coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Structure of the ROC domain from the Parkinson's disease-associated leucine-rich repeat kinase 2 reveals a dimeric GTPase.
Deng J, Lewis PA, Greggio E, Sluch E, Beilina A, Cookson MR
Proc Natl Acad Sci U S A 105 1499-504 (2008)
PMID: 18230735

Function and Biology Details

Reaction catalysed: 
ATP + a protein = ADP + a phosphoprotein
Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-177795 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Leucine-rich repeat serine/threonine-protein kinase 2 Chains: A, B
Molecule details ›
Chains: A, B
Length: 184 amino acids
Theoretical weight: 21.42 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q5S007 (Residues: 1333-1516; Coverage: 7%)
Gene names: LRRK2, PARK8
Sequence domains: Ras of Complex, Roc, domain of DAPkinase
Structure domains:

Ligands and Environments

2 bound ligands:
Ligand MG 2 x MG
Ligand GDP 2 x GDP
1 modified residue:
Ligand MSE 14 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 19-ID
Spacegroup: P1
Unit cell:
a: 44.438Å b: 44.404Å c: 53.681Å
α: 81.51° β: 69° γ: 78.71°
R-values:
R R work R free
0.202 0.199 0.253
Expression system: Escherichia coli

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Citations

52 review citations

Mitochondrial dysfunction in Parkinson's disease: molecular mechanisms and pathophysiological consequences.
Exner et al. (2012)
51 more

22 mentions without citation

Structural biology of the LRRK2 GTPase and kinase domains: implications for regulation.
Gilsbach et al. (2014)
21 more