› 2zgh

X-ray diffraction
2.17Å resolution

Crystal Structure of active granzyme M bound to its product

Released:
DOI: 10.2210/pdb2zgh/pdb
PDB entry 2zgh coloured by chain, front view.
PDB entry 2zgh coloured by chain, side view.
PDB entry 2zgh coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Structural basis for proteolytic specificity of the human apoptosis-inducing granzyme M.
Wu L, Wang L, Hua G, Liu K, Yang X, Zhai Y, Bartlam M, Sun F, Fan Z
J Immunol 183 421-9 (2009)
PMID: 19542453

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-156292 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Granzyme M Chain: A
Molecule details ›
Chain: A
Length: 240 amino acids
Theoretical weight: 26.13 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P51124 (Residues: 26-257; Coverage: 99%)
Gene names: GZMM, MET1
Sequence domains: Trypsin
Structure domains: Trypsin-like serine proteases
SSGKVPL Chain: B
Molecule details ›
Chain: B
Length: 7 amino acids
Theoretical weight: 688 Da
Source organism: Homo sapiens
Expression system: Not provided

Ligands and Environments

1 bound ligand:
Ligand SO4 5 x SO4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU
Spacegroup: P3121
Unit cell:
a: 74.598Å b: 74.598Å c: 112.87Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.214 0.212 0.25
Expression systems:
  • Escherichia coli
  • Not provided

Quick links

Citations

2 review citations

Orphan granzymes find a home.
Bovenschen et al. (2010)
1 more

4 mentions without citation

Solvation Free Energy as a Measure of Hydrophobicity: Application to Serine Protease Binding Interfaces.
Kraml et al. (2019)
3 more