› 2zkm

X-ray diffraction
1.62Å resolution

Crystal Structure of Phospholipase C Beta 2

Released:
DOI: 10.2210/pdb2zkm/pdb
PDB entry 2zkm coloured by chain, front view.
PDB entry 2zkm coloured by chain, side view.
PDB entry 2zkm coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
General and versatile autoinhibition of PLC isozymes.
Hicks SN, Jezyk MR, Gershburg S, Seifert JP, Harden TK, Sondek J
Mol Cell 31 383-94 (2008)
PMID: 18691970

Function and Biology Details

Reaction catalysed: 
1-phosphatidyl-1D-myo-inositol 4,5-bisphosphate + H(2)O = 1D-myo-inositol 1,4,5-trisphosphate + diacylglycerol
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:
Structure domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-169468 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase beta-2 Chain: X
Molecule details ›
Chain: X
Length: 799 amino acids
Theoretical weight: 91.03 KDa
Source organism: Homo sapiens
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: Q00722 (Residues: 1-803; Coverage: 67%)
Gene name: PLCB2
Sequence domains:
Structure domains:

Ligands and Environments

1 bound ligand:
Ligand CA 1 x CA
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 22-ID
Spacegroup: P212121
Unit cell:
a: 80.404Å b: 86.379Å c: 147.447Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.197 0.196 0.213
Expression system: Spodoptera frugiperda

Quick links

Citations

23 review citations

Phosphoinositides: tiny lipids with giant impact on cell regulation.
Balla T. (2013)
22 more

20 mentions without citation

Role of phospholipase Cε in physiological phosphoinositide signaling networks.
Smrcka et al. (2012)
19 more