› 2zme

X-ray diffraction
2.9Å resolution

Integrated structural and functional model of the human ESCRT-II complex

Released:
DOI: 10.2210/pdb2zme/pdb
PDB entry 2zme coloured by chain, front view.
PDB entry 2zme coloured by chain, side view.
PDB entry 2zme coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Integrated structural model and membrane targeting mechanism of the human ESCRT-II complex.
Im YJ, Hurley JH
Dev Cell 14 902-13 (2008)
PMID: 18539118

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-183211 (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
Vacuolar-sorting protein SNF8 Chain: A
Molecule details ›
Chain: A
Length: 258 amino acids
Theoretical weight: 28.9 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q96H20 (Residues: 1-258; Coverage: 100%)
Gene names: EAP30, SNF8
Sequence domains: EAP30/Vps36 family
Structure domains: Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain
Vacuolar protein-sorting-associated protein 36 Chain: B
Molecule details ›
Chain: B
Length: 238 amino acids
Theoretical weight: 26.75 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q86VN1 (Residues: 149-386; Coverage: 62%)
Gene names: C13orf9, CGI-145, EAP45, VPS36
Sequence domains: EAP30/Vps36 family
Structure domains:
Vacuolar protein-sorting-associated protein 25 Chains: C, D
Molecule details ›
Chains: C, D
Length: 102 amino acids
Theoretical weight: 12.24 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q9BRG1 (Residues: 1-102; Coverage: 58%)
Gene names: DERP9, EAP20, VPS25
Sequence domains: ESCRT-II complex subunit
Structure domains: "Winged helix" DNA-binding domain. Chain C. Domain 1

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 22-ID
Spacegroup: P3212
Unit cell:
a: 81.48Å b: 81.48Å c: 226.244Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.219 0.214 0.313
Expression system: Escherichia coli

Quick links

Citations

27 review citations

The ESCRT machinery in endosomal sorting of ubiquitylated membrane proteins.
Raiborg et al. (2009)
26 more

4 mentions without citation

Membrane fission reactions of the mammalian ESCRT pathway.
McCullough et al. (2013)
3 more