› 2zn9

X-ray diffraction
2.4Å resolution

Crystal structure of Ca2+-bound form of des3-20ALG-2

Released:
DOI: 10.2210/pdb2zn9/pdb
PDB entry 2zn9 coloured by chain, front view.
PDB entry 2zn9 coloured by chain, side view.
PDB entry 2zn9 coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Structural basis for Ca2+ -dependent formation of ALG-2/Alix peptide complex: Ca2+/EF3-driven arginine switch mechanism.
Suzuki H, Kawasaki M, Inuzuka T, Okumura M, Kakiuchi T, Shibata H, Wakatsuki S, Maki M
Structure 16 1562-73 (2008)
PMID: 18940611

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-130902 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Programmed cell death protein 6 Chains: A, B
Molecule details ›
Chains: A, B
Length: 172 amino acids
Theoretical weight: 20.16 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: O75340 (Residues: 20-191; Coverage: 90%)
Gene names: ALG2, PDCD6
Sequence domains: EF-hand domain pair
Structure domains: EF-hand

Ligands and Environments

3 bound ligands:
Ligand CA 7 x CA
Ligand 2PE 1 x 2PE
Ligand 12P 1 x 12P
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: PHOTON FACTORY BEAMLINE BL-6A
Spacegroup: P41
Unit cell:
a: 72.024Å b: 72.024Å c: 91.663Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.237 0.234 0.298
Expression system: Escherichia coli

Quick links

Citations

8 review citations

The ESCRT complexes.
Hurley JH. (2010)
7 more

8 mentions without citation

Multifaceted Roles of ALG-2 in Ca(2+)-Regulated Membrane Trafficking.
Maki et al. (2016)
7 more