› 2zpr

X-ray diffraction
1.75Å resolution

Crystal structure of anionic trypsin isoform 2 from chum salmon

Released:
DOI: 10.2210/pdb2zpr/pdb
PDB entry 2zpr coloured by chain, front view.
PDB entry 2zpr coloured by chain, side view.
PDB entry 2zpr coloured by chain, top view.
Source organism: Oncorhynchus keta
Primary publication:
A structural comparison of three isoforms of anionic trypsin from chum salmon (Oncorhynchus keta).
Toyota E, Iyaguchi D, Sekizaki H, Tateyama M, Ng KK
Acta Crystallogr D Biol Crystallogr 65 717-23 (2009)
PMID: 19564692

Function and Biology Details

Reaction catalysed: 
Preferential cleavage: Arg-|-, Lys-|-.
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-109978 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Peptidase S1 domain-containing protein Chains: A, B
Molecule details ›
Chains: A, B
Length: 222 amino acids
Theoretical weight: 23.75 KDa
Source organism: Oncorhynchus keta
UniProt:
  • Canonical: B3Y8K5 (Residues: 1-222; Coverage: 100%)
Sequence domains: Trypsin
Structure domains: Trypsin-like serine proteases

Ligands and Environments

2 bound ligands:
Ligand CA 2 x CA
Ligand BEN 2 x BEN
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU MICROMAX-007
Spacegroup: P212121
Unit cell:
a: 61.381Å b: 75.292Å c: 82.003Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.206 0.206 0.223

Quick links

Citations

2 citation in other articles

Papain-catalyzed peptide bond formation: enzyme-specific activation with guanidinophenyl esters.
de Beer et al. (2011)
1 more

1 mention without citation

Fish trypsins: potential applications in biomedicine and prospects for production.
Jesús-de la Cruz et al. (2018)