› 2zpx

X-ray diffraction
2.83Å resolution

TNF Receptor Subtype One-selective TNF Mutant with Antagonistic Activity; R1antTNF-T8

Released:
DOI: 10.2210/pdb2zpx/pdb
PDB entry 2zpx coloured by chain, front view.
PDB entry 2zpx coloured by chain, side view.
PDB entry 2zpx coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Fast binding kinetics and conserved 3D structure underlie the antagonistic activity of mutant TNF: useful information for designing artificial proteo-antagonists.
Mukai Y, Nakamura T, Yoshioka Y, Shibata H, Abe Y, Nomura T, Taniai M, Ohta T, Nakagawa S, Tsunoda S, Kamada H, Yamagata Y, Tsutsumi Y
J Biochem 146 167-72 (2009)
PMID: 19386778

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-134285 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Tumor necrosis factor, soluble form Chains: A, B, C
Molecule details ›
Chains: A, B, C
Length: 157 amino acids
Theoretical weight: 17.28 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P01375 (Residues: 77-233; Coverage: 67%)
Gene names: TNF, TNFA, TNFSF2
Sequence domains: TNF(Tumour Necrosis Factor) family
Structure domains: Jelly Rolls

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SPRING-8 BEAMLINE BL41XU
Spacegroup: P212121
Unit cell:
a: 49.586Å b: 111.331Å c: 75.382Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.268 0.268 0.309
Expression system: Escherichia coli

Quick links

Citations

3 review citations

Biologics: the next-generation therapeutics for analgesia?
Hatcher et al. (2011)
2 more

4 mentions without citation

Structural pathways of cytokines may illuminate their roles in regulation of cancer development and immunotherapy.
Guven-Maiorov et al. (2014)
3 more