› 2zuc

X-ray diffraction
3.3Å resolution

Crystal structure of left-handed RadA filament

Released:
DOI: 10.2210/pdb2zuc/pdb
PDB entry 2zuc coloured by chain, front view.
PDB entry 2zuc coloured by chain, side view.
PDB entry 2zuc coloured by chain, top view.
Source organism: Saccharolobus solfataricus
Primary publication:
Three new structures of left-handed RADA helical filaments: structural flexibility of N-terminal domain is critical for recombinase activity.
Chang YW, Ko TP, Lee CD, Chang YC, Lin KA, Chang CS, Wang AH, Wang TF
PLoS One 4 e4890 (2009)
PMID: 19295907

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-176262 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
DNA repair and recombination protein RadA Chains: A, B
Molecule details ›
Chains: A, B
Length: 324 amino acids
Theoretical weight: 35.91 KDa
Source organism: Saccharolobus solfataricus
Expression system: Escherichia coli
UniProt:
  • Canonical: Q55075 (Residues: 1-324; Coverage: 100%)
Gene names: SSO0250, radA
Sequence domains:
Structure domains:

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSRRC BEAMLINE BL13B1
Spacegroup: P212121
Unit cell:
a: 47.143Å b: 114.792Å c: 136.084Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.231 0.228 0.283
Expression system: Escherichia coli

Quick links

Citations

3 review citations

XRCC4 and XLF form long helical protein filaments suitable for DNA end protection and alignment to facilitate DNA double strand break repair.
Mahaney et al. (2013)
2 more

2 mentions without citation

Three new structures of left-handed RADA helical filaments: structural flexibility of N-terminal domain is critical for recombinase activity.
Chang et al. (2009)
1 more