› 2zy9

X-ray diffraction
2.94Å resolution

Improved crystal structure of magnesium transporter MgtE

Released:
DOI: 10.2210/pdb2zy9/pdb
PDB entry 2zy9 coloured by chain, front view.
PDB entry 2zy9 coloured by chain, side view.
PDB entry 2zy9 coloured by chain, top view.
Source organism: Thermus thermophilus HB8
Primary publication:
Mg(2+)-dependent gating of bacterial MgtE channel underlies Mg(2+) homeostasis.
Hattori M, Iwase N, Furuya N, Tanaka Y, Tsukazaki T, Ishitani R, Maguire ME, Ito K, Maturana A, Nureki O
EMBO J 28 3602-12 (2009)
PMID: 19798051

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-178266 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Magnesium transporter MgtE Chains: A, B
Molecule details ›
Chains: A, B
Length: 473 amino acids
Theoretical weight: 52.49 KDa
Source organism: Thermus thermophilus HB8
Expression system: Escherichia coli
UniProt:
  • Canonical: Q5SMG8 (Residues: 1-450; Coverage: 100%)
Gene names: TTHA1060, mgtE
Sequence domains:
Structure domains:

Ligands and Environments

1 bound ligand:
Ligand MG 13 x MG
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SPRING-8 BEAMLINE BL41XU
Spacegroup: C2
Unit cell:
a: 135.364Å b: 76.527Å c: 160.037Å
α: 90° β: 102.17° γ: 90°
R-values:
R R work R free
0.257 0.255 0.295
Expression system: Escherichia coli

Quick links

Citations

12 review citations

Metal homeostasis and resistance in bacteria.
Chandrangsu et al. (2017)
11 more

12 mentions without citation

Structural and functional comparison of magnesium transporters throughout evolution.
Franken et al. (2022)
11 more