› 3a4u

X-ray diffraction
1.84Å resolution

Crystal structure of MCFD2 in complex with carbohydrate recognition domain of ERGIC-53

Released:
DOI: 10.2210/pdb3a4u/pdb
PDB entry 3a4u coloured by chain, front view.
PDB entry 3a4u coloured by chain, side view.
PDB entry 3a4u coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Structural basis for the cooperative interplay between the two causative gene products of combined factor V and factor VIII deficiency.
Nishio M, Kamiya Y, Mizushima T, Wakatsuki S, Sasakawa H, Yamamoto K, Uchiyama S, Noda M, McKay AR, Fukui K, Hauri HP, Kato K
Proc Natl Acad Sci U S A 107 4034-9 (2010)
PMID: 20142513

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-155904 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Protein ERGIC-53 Chain: A
Molecule details ›
Chain: A
Length: 255 amino acids
Theoretical weight: 28.04 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P49257 (Residues: 31-285; Coverage: 53%)
Gene names: ERGIC53, F5F8D, LMAN1
Sequence domains: Legume-like lectin family
Structure domains: Jelly Rolls
Multiple coagulation factor deficiency protein 2 Chain: B
Molecule details ›
Chain: B
Length: 143 amino acids
Theoretical weight: 16.32 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q8NI22 (Residues: 27-146; Coverage: 100%)
Gene names: MCFD2, SDNSF
Sequence domains: EF-hand domain pair
Structure domains: EF-hand

Ligands and Environments

2 bound ligands:
Ligand CA 4 x CA
Ligand GOL 4 x GOL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: PHOTON FACTORY BEAMLINE BL-5A
Spacegroup: P3121
Unit cell:
a: 59.41Å b: 59.41Å c: 198.842Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.188 0.188 0.2
Expression system: Escherichia coli

Quick links

Citations

10 review citations

Protein secretion and the endoplasmic reticulum.
Benham AM. (2012)
9 more

5 mentions without citation

Structural characterization of carbohydrate binding by LMAN1 protein provides new insight into the endoplasmic reticulum export of factors V (FV) and VIII (FVIII).
Zheng et al. (2013)
4 more