› 3aaf

X-ray diffraction
1.9Å resolution

Structure of WRN RQC domain bound to double-stranded DNA

Released:
DOI: 10.2210/pdb3aaf/pdb
PDB entry 3aaf coloured by chain, front view.
PDB entry 3aaf coloured by chain, side view.
PDB entry 3aaf coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Structural basis for DNA strand separation by the unconventional winged-helix domain of RecQ helicase WRN.
Kitano K, Kim SY, Hakoshima T
Structure 18 177-87 (2010)
PMID: 20159463

Function and Biology Details

Reaction catalysed: 
ATP + H(2)O = ADP + phosphate
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-116061 (preferred)
Entry contents:
1 distinct polypeptide molecule
1 distinct DNA molecule
Macromolecules (2 distinct):
Bifunctional 3'-5' exonuclease/ATP-dependent helicase WRN Chains: A, B
Molecule details ›
Chains: A, B
Length: 134 amino acids
Theoretical weight: 15.35 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q14191 (Residues: 949-1079; Coverage: 9%)
Gene names: RECQ3, RECQL2, WRN
Sequence domains: RQC domain
Structure domains: Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain
DNA (5'-D(*AP*CP*CP*CP*TP*AP*AP*TP*TP*AP*GP*GP*GP*T)-3') Chains: C, D
Molecule details ›
Chains: C, D
Length: 14 nucleotides
Theoretical weight: 4.28 KDa
Source organism: Homo sapiens
Expression system: Not provided

Ligands and Environments

1 bound ligand:
Ligand ACT 2 x ACT
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SPRING-8 BEAMLINE BL44XU, SPRING-8 BEAMLINE BL41XU
Spacegroup: P4322
Unit cell:
a: 84.077Å b: 84.077Å c: 145.291Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.213 0.212 0.244
Expression systems:
  • Escherichia coli
  • Not provided

Quick links

Citations

19 review citations

DNA methyltransferases, DNA damage repair, and cancer.
Jin et al. (2013)
18 more

13 mentions without citation

Human RecQ helicases in DNA repair, recombination, and replication.
Croteau et al. (2014)
12 more