› 3abe

X-ray diffraction
2.6Å resolution

Structure of human REV7 in complex with a human REV3 fragment in a tetragonal crystal

Released:
DOI: 10.2210/pdb3abe/pdb
PDB entry 3abe coloured by chain, front view.
PDB entry 3abe coloured by chain, side view.
PDB entry 3abe coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Crystal structure of human REV7 in complex with a human REV3 fragment and structural implication of the interaction between DNA polymerase zeta and REV1.
Hara K, Hashimoto H, Murakumo Y, Kobayashi S, Kogame T, Unzai S, Akashi S, Takeda S, Shimizu T, Sato M
J Biol Chem 285 12299-307 (2010)
PMID: 20164194

Function and Biology Details

Reaction catalysed: 
Deoxynucleoside triphosphate + DNA(n) = diphosphate + DNA(n+1)
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-130062 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Mitotic spindle assembly checkpoint protein MAD2B Chain: C
Molecule details ›
Chain: C
Length: 227 amino acids
Theoretical weight: 26.1 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q9UI95 (Residues: 1-211; Coverage: 100%)
Gene names: MAD2B, MAD2L2, REV7
Sequence domains: HORMA domain
Structure domains: HORMA domain
DNA polymerase zeta catalytic subunit Chain: Z
Molecule details ›
Chain: Z
Length: 52 amino acids
Theoretical weight: 5.63 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: O60673 (Residues: 1847-1898; Coverage: 2%)
Gene names: POLZ, REV3, REV3L

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: PHOTON FACTORY BEAMLINE BL-5A
Spacegroup: P41212
Unit cell:
a: 76.517Å b: 76.517Å c: 118.524Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.242 0.24 0.281
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

24 review citations

Regulation of DNA cross-link repair by the Fanconi anemia/BRCA pathway.
Kim et al. (2012)
23 more

7 mentions without citation

Fanconi anemia pathway as a prospective target for cancer intervention.
Liu et al. (2020)
6 more