› 3adl

X-ray diffraction
2.2Å resolution

Structure of TRBP2 and its molecule implications for miRNA processing

Released:
DOI: 10.2210/pdb3adl/pdb
PDB entry 3adl coloured by chain, front view.
PDB entry 3adl coloured by chain, side view.
PDB entry 3adl coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Structure of Arabidopsis HYPONASTIC LEAVES1 and its molecular implications for miRNA processing.
Yang SW, Chen HY, Yang J, Machida S, Chua NH, Yuan YA
Structure 18 594-605 (2010)
PMID: 20462493

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-172351 (preferred)
Entry contents:
1 distinct polypeptide molecule
1 distinct RNA molecule
Macromolecules (2 distinct):
RISC-loading complex subunit TARBP2 Chain: A
Molecule details ›
Chain: A
Length: 88 amino acids
Theoretical weight: 9.93 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q15633 (Residues: 161-231; Coverage: 19%)
Gene names: TARBP2, TRBP
Sequence domains: Double-stranded RNA binding motif
Structure domains: Double Stranded RNA Binding Domain
RNA (5'-R(P*CP*GP*CP*GP*CP*GP*CP*GP*CP*G)-3') Chains: B, C
Molecule details ›
Chains: B, C
Length: 10 nucleotides
Theoretical weight: 3.21 KDa
Source organism: Homo sapiens
Expression system: Not provided

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X12C
Spacegroup: I212121
Unit cell:
a: 55.3Å b: 60.425Å c: 99.892Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.263 0.261 0.298
Expression systems:
  • Escherichia coli
  • Not provided

Quick links

Citations

21 review citations

Biogenesis, turnover, and mode of action of plant microRNAs.
Rogers et al. (2013)
20 more

23 mentions without citation

Molecular mechanisms of RNA interference.
Wilson et al. (2013)
22 more