› 3adw

X-ray diffraction
2.07Å resolution

Human PPARgamma ligand-binding domain in complex with 5-methoxy-indole acetate and 15-oxo-eicosatetraenoic acid

Released:
DOI: 10.2210/pdb3adw/pdb
PDB entry 3adw coloured by chain, front view.
PDB entry 3adw coloured by chain, side view.
PDB entry 3adw coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
The nuclear receptor PPARγ individually responds to serotonin- and fatty acid-metabolites.
Waku T, Shiraki T, Oyama T, Maebara K, Nakamori R, Morikawa K
EMBO J 29 3395-407 (2010)
PMID: 20717101

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-153379 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Peroxisome proliferator-activated receptor gamma Chains: A, B
Molecule details ›
Chains: A, B
Length: 287 amino acids
Theoretical weight: 32.69 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P37231 (Residues: 223-505; Coverage: 56%)
Gene names: NR1C3, PPARG
Sequence domains: Ligand-binding domain of nuclear hormone receptor
Structure domains: Retinoid X Receptor

Ligands and Environments

2 bound ligands:
Ligand MYI 1 x MYI
Ligand OCR 2 x OCR
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SPRING-8 BEAMLINE BL38B1
Spacegroup: C2
Unit cell:
a: 93.034Å b: 61.181Å c: 118.475Å
α: 90° β: 102.84° γ: 90°
R-values:
R R work R free
0.23 0.23 0.265
Expression system: Escherichia coli

Quick links

Citations

20 review citations

Endogenous ligands for nuclear receptors: digging deeper.
Schupp et al. (2010)
19 more

4 mentions without citation

An alternate binding site for PPARγ ligands.
Hughes et al. (2014)
3 more