› 3ajb

X-ray diffraction
2.5Å resolution

Crystal Structure of human Pex3p in complex with N-terminal Pex19p peptide

Released:
DOI: 10.2210/pdb3ajb/pdb
PDB entry 3ajb coloured by chain, front view.
PDB entry 3ajb coloured by chain, side view.
PDB entry 3ajb coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Structural basis for docking of peroxisomal membrane protein carrier Pex19p onto its receptor Pex3p.
Sato Y, Shibata H, Nakatsu T, Nakano H, Kashiwayama Y, Imanaka T, Kato H
EMBO J 29 4083-93 (2010)
PMID: 21102411

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
Cellular component:
Sequence domain:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-154198 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Peroxisomal biogenesis factor 3 Chain: A
Molecule details ›
Chain: A
Length: 330 amino acids
Theoretical weight: 36.91 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P56589 (Residues: 49-373; Coverage: 87%)
Gene name: PEX3
Sequence domains: Peroxin-3
Peroxisomal biogenesis factor 19 Chain: B
Molecule details ›
Chain: B
Length: 49 amino acids
Theoretical weight: 4.9 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P40855 (Residues: 1-44; Coverage: 15%)
Gene names: HK33, OK/SW-cl.22, PEX19, PXF

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SPRING-8 BEAMLINE BL41XU, SPRING-8 BEAMLINE BL38B1
Spacegroup: P6522
Unit cell:
a: 147.6Å b: 147.6Å c: 86.202Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.221 0.219 0.254
Expression system: Escherichia coli

Quick links

Citations

14 review citations

Multiple pathways for protein transport to peroxisomes.
Kim et al. (2015)
13 more

5 mentions without citation

Mechanisms of Tail-Anchored Membrane Protein Targeting and Insertion.
Chio et al. (2017)
4 more