› 3al2

X-ray diffraction
2Å resolution

Crystal Structure of TopBP1 BRCT7/8

Released:
DOI: 10.2210/pdb3al2/pdb
PDB entry 3al2 coloured by chain, front view.
PDB entry 3al2 coloured by chain, side view.
PDB entry 3al2 coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Molecular basis of BACH1/FANCJ recognition by TopBP1 in DNA replication checkpoint control.
Leung CC, Gong Z, Chen J, Glover JN
J Biol Chem 286 4292-301 (2011)
PMID: 21127055

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-187682 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
DNA topoisomerase 2-binding protein 1 Chain: A
Molecule details ›
Chain: A
Length: 235 amino acids
Theoretical weight: 26.57 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q92547 (Residues: 1264-1493; Coverage: 15%)
Gene names: KIAA0259, TOPBP1
Sequence domains: BRCA1 C Terminus (BRCT) domain
Structure domains: BRCT domain

Ligands and Environments

1 bound ligand:
Ligand SO4 2 x SO4
1 modified residue:
Ligand MSE 2 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: CLSI BEAMLINE 08ID-1
Spacegroup: C2
Unit cell:
a: 102.583Å b: 32.748Å c: 81.762Å
α: 90° β: 109.32° γ: 90°
R-values:
R R work R free
0.172 0.17 0.204
Expression system: Escherichia coli

Quick links

Citations

10 review citations

ATR signalling: more than meeting at the fork.
Nam et al. (2011)
9 more

6 mentions without citation

BRCT domains: easy as one, two, three.
Leung et al. (2011)
5 more