3b0c

X-ray diffraction
2.2Å resolution

Crystal structure of the chicken CENP-T histone fold/CENP-W complex, crystal form I

Released:

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
PDBe Complex ID:
PDB-CPX-123325 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Centromere protein T Chain: T
Molecule details ›
Chain: T
Length: 111 amino acids
Theoretical weight: 12.75 KDa
Source organism: Gallus gallus
Expression system: Escherichia coli
UniProt:
  • Canonical: F1NPG5 (Residues: 531-639; Coverage: 17%)
Gene name: CENPT
Sequence domains: Centromere kinetochore component CENP-T histone fold
Structure domains: Histone, subunit A
Centromere protein W Chain: W
Molecule details ›
Chain: W
Length: 76 amino acids
Theoretical weight: 8.69 KDa
Source organism: Gallus gallus
Expression system: Escherichia coli
UniProt:
  • Canonical: P0DJH6 (Residues: 2-76; Coverage: 99%)
Gene name: CENPW
Sequence domains: CENP-W protein
Structure domains: Histone, subunit A

Ligands and Environments

1 bound ligand:
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SPRING-8 BEAMLINE BL38B1
Spacegroup: P3221
Unit cell:
a: 54.933Å b: 54.933Å c: 122.463Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.229 0.22 0.269
Expression system: Escherichia coli