› 3b76

X-ray diffraction
1.75Å resolution

Crystal structure of the third PDZ domain of human ligand-of-numb protein-X (LNX1) in complex with the C-terminal peptide from the coxsackievirus and adenovirus receptor

Released:
DOI: 10.2210/pdb3b76/pdb
PDB entry 3b76 coloured by chain, front view.
PDB entry 3b76 coloured by chain, side view.
PDB entry 3b76 coloured by chain, top view.
Source organism: Homo sapiens
Entry authors: Ugochukwu E, Burgess-Brown N, Berridge G, Elkins J, Bunkoczi G, Pike ACW, Sundstrom M, Arrowsmith CH, Weigelt J, Edwards AM, Gileadi O, von Delft F, Doyle D, Structural Genomics Consortium (SGC)

Function and Biology Details

Reaction catalysed: 
S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + N(6)-ubiquitinyl-[acceptor protein]-L-lysine
Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-186172 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
E3 ubiquitin-protein ligase LNX Chains: A, B
Molecule details ›
Chains: A, B
Length: 118 amino acids
Theoretical weight: 12.82 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q8TBB1 (Residues: 504-594; Coverage: 13%)
Gene names: LNX, LNX1, PDZRN2, UNQ574/PRO1136
Sequence domains: PDZ domain
Structure domains: Pdz3 Domain

Ligands and Environments

2 bound ligands:
Ligand NA 1 x NA
Ligand EDO 2 x EDO
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SLS BEAMLINE X10SA
Spacegroup: P212121
Unit cell:
a: 49.782Å b: 58.449Å c: 73.741Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.177 0.175 0.205
Expression system: Escherichia coli

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