› 3b7o

X-ray diffraction
1.6Å resolution

Crystal structure of the human tyrosine phosphatase SHP2 (PTPN11) with an accessible active site

Released:
DOI: 10.2210/pdb3b7o/pdb
PDB entry 3b7o coloured by chain, front view.
PDB entry 3b7o coloured by chain, side view.
PDB entry 3b7o coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Large-scale structural analysis of the classical human protein tyrosine phosphatome.
Barr AJ, Ugochukwu E, Lee WH, King ON, Filippakopoulos P, Alfano I, Savitsky P, Burgess-Brown NA, Müller S, Knapp S
Cell 136 352-63 (2009)
PMID: 19167335

Function and Biology Details

Reaction catalysed: 
Protein tyrosine phosphate + H(2)O = protein tyrosine + phosphate
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-170287 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Tyrosine-protein phosphatase non-receptor type 11 Chain: A
Molecule details ›
Chain: A
Length: 316 amino acids
Theoretical weight: 36.88 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q06124 (Residues: 237-529; Coverage: 49%)
Gene names: PTP2C, PTPN11, SHPTP2
Sequence domains: Protein-tyrosine phosphatase
Structure domains: Protein tyrosine phosphatase superfamily

Ligands and Environments

1 bound ligand:
Ligand MLT 1 x MLT
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SLS BEAMLINE X10SA
Spacegroup: C2221
Unit cell:
a: 44.813Å b: 86.496Å c: 166.555Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.172 0.17 0.209
Expression system: Escherichia coli

Quick links

Citations

57 review citations

Inside the human cancer tyrosine phosphatome.
Julien et al. (2011)
56 more

26 mentions without citation

Targeting protein tyrosine phosphatases for anticancer drug discovery.
Scott et al. (2010)
25 more