› 3bhd

X-ray diffraction
1.5Å resolution

Crystal structure of human thiamine triphosphatase (THTPA)

Released:
DOI: 10.2210/pdb3bhd/pdb
PDB entry 3bhd coloured by chain, front view.
PDB entry 3bhd coloured by chain, side view.
PDB entry 3bhd coloured by chain, top view.
Source organism: Homo sapiens
Entry authors: Busam RD, Lehtio L, Arrowsmith CH, Collins R, Dahlgren LG, Edwards AM, Flodin S, Flores A, Graslund S, Hammarstrom M, Hallberg BM, Herman MD, Johansson A, Johansson I, Kallas A, Karlberg T, Kotenyova T, Moche M, Nilsson ME, Nordlund P, Nyman T, Persson C, Sagemark J, Sundstrom M, Svensson L, Thorsell AG, Tresaugues L, Van den Berg S, Weigelt J, Welin M, Berglund H, Structural Genomics Consortium (SGC)

Function and Biology Details

Reaction catalysed: 
Thiamine triphosphate + H(2)O = thiamine diphosphate + phosphate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-189727 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Thiamine-triphosphatase Chains: A, B
Molecule details ›
Chains: A, B
Length: 234 amino acids
Theoretical weight: 26.05 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q9BU02 (Residues: 1-215; Coverage: 94%)
Gene name: THTPA
Sequence domains: CYTH domain
Structure domains: Hypothetical Protein Pfu-838710-001

Ligands and Environments

5 bound ligands:
Ligand SO4 1 x SO4
Ligand CL 2 x CL
Ligand CIT 3 x CIT
Ligand NA 1 x NA
Ligand GOL 5 x GOL
1 modified residue:
Ligand MSE 8 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID14-2
Spacegroup: P21
Unit cell:
a: 60.77Å b: 46.81Å c: 71.87Å
α: 90° β: 113.97° γ: 90°
R-values:
R R work R free
0.178 0.176 0.201
Expression system: Escherichia coli

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