3bi7

X-ray diffraction
1.7Å resolution

Crystal structure of the SRA domain of E3 ubiquitin-protein ligase UHRF1

Released:

Function and Biology Details

Reaction catalysed:
S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + N(6)-ubiquitinyl-[acceptor protein]-L-lysine
Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
monomeric (preferred)
PDBe Complex ID:
PDB-CPX-188767 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
E3 ubiquitin-protein ligase UHRF1 Chain: A
Molecule details ›
Chain: A
Length: 212 amino acids
Theoretical weight: 23.91 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q96T88 (Residues: 414-617; Coverage: 26%)
Gene names: ICBP90, NP95, RNF106, UHRF1
Sequence domains: SAD/SRA domain
Structure domains: SRA-YDG

Ligands and Environments

3 bound ligands:
1 modified residue:

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 23-ID-B
Spacegroup: P3121
Unit cell:
a: 65.975Å b: 65.975Å c: 96.061Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.16 0.159 0.197
Expression system: Escherichia coli BL21(DE3)