› 3bjf

X-ray diffraction
2.03Å resolution

Pyruvate kinase M2 is a phosphotyrosine binding protein

Released:
DOI: 10.2210/pdb3bjf/pdb
PDB entry 3bjf coloured by chain, front view.
PDB entry 3bjf coloured by chain, side view.
PDB entry 3bjf coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Pyruvate kinase M2 is a phosphotyrosine-binding protein.
Christofk HR, Vander Heiden MG, Wu N, Asara JM, Cantley LC
Nature 452 181-6 (2008)
PMID: 18337815

Function and Biology Details

Reactions catalysed: 
ATP + pyruvate = ADP + phosphoenolpyruvate
ATP + a [protein]-L-tyrosine = ADP + a [protein]-L-tyrosine phosphate
ATP + a protein = ADP + a phosphoprotein
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-146985 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Pyruvate kinase PKM Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 518 amino acids
Theoretical weight: 56.63 KDa
Source organism: Homo sapiens
UniProt:
  • Canonical: P14618 (Residues: 14-531; Coverage: 98%)
Gene names: OIP3, PK2, PK3, PKM, PKM2
Sequence domains:
Structure domains:

Ligands and Environments

4 bound ligands:
Ligand FBP 4 x FBP
Ligand OXL 4 x OXL
Ligand K 4 x K
Ligand MG 4 x MG
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 24-ID-C
Spacegroup: P1
Unit cell:
a: 80.756Å b: 81.458Å c: 106.885Å
α: 74.63° β: 69.91° γ: 66.1°
R-values:
R R work R free
0.208 0.208 0.246

Quick links

Citations

230 review citations

Understanding the Warburg effect: the metabolic requirements of cell proliferation.
Vander Heiden et al. (2009)
229 more

11 mentions without citation

Pyruvate kinase: Function, regulation and role in cancer.
Israelsen et al. (2015)
10 more