› 3bji

X-ray diffraction
2.6Å resolution

Structural Basis of Promiscuous Guanine Nucleotide Exchange by the T-Cell Essential Vav1

Released:
DOI: 10.2210/pdb3bji/pdb
PDB entry 3bji coloured by chain, front view.
PDB entry 3bji coloured by chain, side view.
PDB entry 3bji coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Structural basis of guanine nucleotide exchange mediated by the T-cell essential Vav1.
Chrencik JE, Brooun A, Zhang H, Mathews II, Hura GL, Foster SA, Perry JJ, Streiff M, Ramage P, Widmer H, Bokoch GM, Tainer JA, Weckbecker G, Kuhn P
J Mol Biol 380 828-43 (2008)
PMID: 18589439

Function and Biology Details

Reaction catalysed: 
GTP + H(2)O = GDP + phosphate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-147391 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Proto-oncogene vav Chains: A, B
Molecule details ›
Chains: A, B
Length: 378 amino acids
Theoretical weight: 44.49 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P15498 (Residues: 189-565; Coverage: 45%)
Gene names: VAV, VAV1
Sequence domains:
Structure domains:
Ras-related C3 botulinum toxin substrate 1 Chains: C, D
Molecule details ›
Chains: C, D
Length: 177 amino acids
Theoretical weight: 19.71 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P63000 (Residues: 1-177; Coverage: 92%)
Gene names: MIG5, RAC1, TC25
Sequence domains: Ras family
Structure domains: P-loop containing nucleotide triphosphate hydrolases

Ligands and Environments

1 bound ligand:
Ligand ZN 4 x ZN
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 23-ID-B
Spacegroup: P21
Unit cell:
a: 78.948Å b: 75.079Å c: 114.857Å
α: 90° β: 103.87° γ: 90°
R-values:
R R work R free
0.225 0.221 0.293
Expression system: Escherichia coli

Quick links

Citations

7 review citations

Regulation of small GTPases by GEFs, GAPs, and GDIs.
Cherfils et al. (2013)
6 more

10 mentions without citation

Structural Insights into the Regulation Mechanism of Small GTPases by GEFs.
Toma-Fukai et al. (2019)
9 more