› 3bo8

X-ray diffraction
1.8Å resolution

The High Resolution Crystal Structure of HLA-A1 Complexed with the MAGE-A1 Peptide

Released:
DOI: 10.2210/pdb3bo8/pdb
PDB entry 3bo8 coloured by chain, front view.
PDB entry 3bo8 coloured by chain, side view.
PDB entry 3bo8 coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Conformational changes within the HLA-A1:MAGE-A1 complex induced by binding of a recombinant antibody fragment with TCR-like specificity.
Kumar P, Vahedi-Faridi A, Saenger W, Ziegler A, Uchanska-Ziegler B
Protein Sci 18 37-49 (2009)
PMID: 19177349

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
PDBe Complex ID:
PDB-CPX-137694 (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
HLA class I histocompatibility antigen, A alpha chain Chain: A
Molecule details ›
Chain: A
Length: 274 amino acids
Theoretical weight: 31.64 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P04439 (Residues: 25-298; Coverage: 80%)
Gene names: HLA-A, HLAA
Sequence domains:
Structure domains:
Beta-2-microglobulin Chain: B
Molecule details ›
Chain: B
Length: 100 amino acids
Theoretical weight: 11.88 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P61769 (Residues: 21-119; Coverage: 100%)
Gene names: B2M, CDABP0092, HDCMA22P
Sequence domains: Immunoglobulin C1-set domain
Structure domains: Immunoglobulins
Melanoma-associated antigen 1 Chain: C
Molecule details ›
Chain: C
Length: 9 amino acids
Theoretical weight: 977 Da
Source organism: Homo sapiens
Expression system: Not provided
UniProt:
  • Canonical: P43355 (Residues: 161-169; Coverage: 3%)
Gene names: MAGE1, MAGE1A, MAGEA1

Ligands and Environments

1 bound ligand:
Ligand GOL 3 x GOL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: BESSY BEAMLINE 14.1
Spacegroup: P212121
Unit cell:
a: 51.176Å b: 74.06Å c: 125.94Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.196 0.195 0.225
Expression systems:
  • Escherichia coli BL21(DE3)
  • Escherichia coli
  • Not provided

Quick links

Citations

2 review citations

Opportunities and Challenges for Antibodies against Intracellular Antigens.
Yang et al. (2019)
1 more

18 mentions without citation

Crystal structure of swine major histocompatibility complex class I SLA-1 0401 and identification of 2009 pandemic swine-origin influenza A H1N1 virus cytotoxic T lymphocyte epitope peptides.
Zhang et al. (2011)
17 more