› 3bsq

X-ray diffraction
2.8Å resolution

Crystal structure of human kallikrein 7 produced as a secretion protein in E.coli

Released:
DOI: 10.2210/pdb3bsq/pdb
PDB entry 3bsq coloured by chain, front view.
PDB entry 3bsq coloured by chain, side view.
PDB entry 3bsq coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Crystal structure of human epidermal kallikrein 7 (hK7) synthesized directly in its native state in E. coli: insights into the atomic basis of its inhibition by LEKTI domain 6 (LD6).
Fernández IS, Ständker L, Mägert HJ, Forssmann WG, Giménez-Gallego G, Romero A
J Mol Biol 377 1488-97 (2008)
PMID: 18329042

Function and Biology Details

Reaction catalysed: 
Cleavage of proteins with aromatic side chains in the P1 position
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-156068 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Kallikrein-7 Chains: A, B, C
Molecule details ›
Chains: A, B, C
Length: 227 amino acids
Theoretical weight: 24.89 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P49862 (Residues: 30-250; Coverage: 96%)
Gene names: KLK7, PRSS6, SCCE
Sequence domains: Trypsin
Structure domains: Trypsin-like serine proteases

Ligands and Environments

1 bound ligand:
Ligand SO4 16 x SO4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID14-2
Spacegroup: R32
Unit cell:
a: 113.58Å b: 113.58Å c: 326.39Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.217 0.217 0.292
Expression system: Escherichia coli

Quick links

Citations

3 review citations

The emerging roles of serine protease cascades in the epidermis.
Ovaere et al. (2009)
2 more

4 mentions without citation

Natural and synthetic inhibitors of kallikrein-related peptidases (KLKs).
Goettig et al. (2010)
3 more