› 3bvo

X-ray diffraction
3Å resolution

Crystal structure of human co-chaperone protein HscB

Released:
DOI: 10.2210/pdb3bvo/pdb
PDB entry 3bvo coloured by chain, front view.
PDB entry 3bvo coloured by chain, side view.
PDB entry 3bvo coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Structure of human J-type co-chaperone HscB reveals a tetracysteine metal-binding domain.
Bitto E, Bingman CA, Bittova L, Kondrashov DA, Bannen RM, Fox BG, Markley JL, Phillips GN
J Biol Chem 283 30184-92 (2008)
PMID: 18713742

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-184888 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Iron-sulfur cluster co-chaperone protein HscB, mitochondrial Chains: A, B
Molecule details ›
Chains: A, B
Length: 207 amino acids
Theoretical weight: 24.4 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q8IWL3 (Residues: 30-235; Coverage: 88%)
Gene names: DNAJC20, HSC20, HSCB
Sequence domains:
Structure domains:

Ligands and Environments

2 bound ligands:
Ligand ZN 2 x ZN
Ligand SO4 1 x SO4
1 modified residue:
Ligand MSE 10 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 23-ID-D
Spacegroup: P21
Unit cell:
a: 63.644Å b: 32.581Å c: 114.362Å
α: 90° β: 105.24° γ: 90°
R-values:
R R work R free
0.24 0.236 0.288
Expression system: Escherichia coli

Quick links

Citations

12 review citations

Biogenesis of iron-sulfur clusters in mammalian cells: new insights and relevance to human disease.
Rouault TA. (2012)
11 more

8 mentions without citation

Tangled web of interactions among proteins involved in iron-sulfur cluster assembly as unraveled by NMR, SAXS, chemical crosslinking, and functional studies.
Kim et al. (2015)
7 more