› 3bz4

X-ray diffraction
1.8Å resolution

Crystal structure of Fab F22-4 in complex with a Shigella flexneri 2a O-Ag decasaccharide

Released:
DOI: 10.2210/pdb3bz4/pdb
PDB entry 3bz4 coloured by chain, front view.
PDB entry 3bz4 coloured by chain, side view.
PDB entry 3bz4 coloured by chain, top view.
Source organism: Mus musculus
Primary publication:
Structures of synthetic O-antigen fragments from serotype 2a Shigella flexneri in complex with a protective monoclonal antibody.
Vulliez-Le Normand B, Saul FA, Phalipon A, Bélot F, Guerreiro C, Mulard LA, Bentley GA
Proc Natl Acad Sci U S A 105 9976-81 (2008)
PMID: 18621718

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-234855 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (3 distinct):
Fab F22-4 light chain Chains: A, C, E, G
Molecule details ›
Chains: A, C, E, G
Length: 219 amino acids
Theoretical weight: 24.14 KDa
Source organism: Mus musculus
Structure domains: Immunoglobulins
Fab F22-4 heavy chain Chains: B, D, F, H
Molecule details ›
Chains: B, D, F, H
Length: 217 amino acids
Theoretical weight: 23.63 KDa
Source organism: Mus musculus
Structure domains: Immunoglobulins

Ligands and Environments

Carbohydrate polymer : NEW Components: EAG, RAM, NAG, GLC
Ligand EAG 4 x EAG
Ligand RAM 24 x RAM
Ligand NAG 4 x NAG
Ligand GLC 8 x GLC
1 bound ligand:
Ligand PD 1 x PD
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID14-2
Spacegroup: P21
Unit cell:
a: 67.06Å b: 137.6Å c: 109.8Å
α: 90° β: 95.15° γ: 90°
R-values:
R R work R free
0.177 0.176 0.23

Quick links

Citations

15 review citations

Synthesis of bacterial polysaccharides via the Wzx/Wzy-dependent pathway.
Islam et al. (2014)
14 more

2 mentions without citation

Structures of synthetic O-antigen fragments from serotype 2a Shigella flexneri in complex with a protective monoclonal antibody.
Vulliez-Le Normand et al. (2008)
1 more