› 3bzf

X-ray diffraction
2.5Å resolution

The human non-classical major histocompatibility complex molecule HLA-E

Released:
DOI: 10.2210/pdb3bzf/pdb
PDB entry 3bzf coloured by chain, front view.
PDB entry 3bzf coloured by chain, side view.
PDB entry 3bzf coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Subtle changes in peptide conformation profoundly affect recognition of the non-classical MHC class I molecule HLA-E by the CD94-NKG2 natural killer cell receptors.
Hoare HL, Sullivan LC, Clements CS, Ely LK, Beddoe T, Henderson KN, Lin J, Reid HH, Brooks AG, Rossjohn J
J Mol Biol 377 1297-303 (2008)
PMID: 18339401

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domains:

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
PDBe Complex ID:
PDB-CPX-145268 (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
Soluble HLA class I histocompatibility antigen, alpha chain E Chains: A, C
Molecule details ›
Chains: A, C
Length: 276 amino acids
Theoretical weight: 31.92 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P13747 (Residues: 22-297; Coverage: 82%)
Gene names: HLA-6.2, HLA-E, HLAE
Sequence domains:
Structure domains:
Beta-2-microglobulin Chains: B, D
Molecule details ›
Chains: B, D
Length: 97 amino acids
Theoretical weight: 11.51 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P61769 (Residues: 23-119; Coverage: 98%)
Gene names: B2M, CDABP0092, HDCMA22P
Sequence domains: Immunoglobulin C1-set domain
Structure domains: Immunoglobulins
HLA class I histocompatibility antigen, C alpha chain Chains: P, Q
Molecule details ›
Chains: P, Q
Length: 9 amino acids
Theoretical weight: 984 Da
Source organism: Homo sapiens
Expression system: Not provided
UniProt:
  • Canonical: P10321 (Residues: 3-11; Coverage: 0%)
Gene names: HLA-C, HLAC

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 14-BM-C
Spacegroup: P21
Unit cell:
a: 79.52Å b: 62.301Å c: 98.769Å
α: 90° β: 106.15° γ: 90°
R-values:
R R work R free
0.204 0.2 0.269
Expression systems:
  • Escherichia coli
  • Not provided

Quick links

Citations

16 review citations

The adaptable major histocompatibility complex (MHC) fold: structure and function of nonclassical and MHC class I-like molecules.
Adams et al. (2013)
15 more

3 mentions without citation

SIV-induced terminally differentiated adaptive NK cells in lymph nodes associated with enhanced MHC-E restricted activity.
Huot et al. (2021)
2 more