› 3bzh

X-ray diffraction
1.6Å resolution

Crystal structure of human ubiquitin-conjugating enzyme E2 E1

Released:
DOI: 10.2210/pdb3bzh/pdb
PDB entry 3bzh coloured by chain, front view.
PDB entry 3bzh coloured by chain, side view.
PDB entry 3bzh coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
A human ubiquitin conjugating enzyme (E2)-HECT E3 ligase structure-function screen.
Sheng Y, Hong JH, Doherty R, Srikumar T, Shloush J, Avvakumov GV, Walker JR, Xue S, Neculai D, Wan JW, Kim SK, Arrowsmith CH, Raught B, Dhe-Paganon S
Mol Cell Proteomics 11 329-41 (2012)
PMID: 22496338

Function and Biology Details

Reactions catalysed: 
S-ubiquitinyl-[E1 ubiquitin-activating enzyme]-L-cysteine + [E2 ubiquitin-conjugating enzyme]-L-cysteine = [E1 ubiquitin-activating enzyme]-L-cysteine + S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine
(1a) S-ubiquitinyl-[E1 ubiquitin-activating enzyme]-L-cysteine + [(E3-independent) E2 ubiquitin-conjugating enzyme]-L-cysteine = [E1 ubiquitin-activating enzyme]-L-cysteine + S-monoubiquitinyl-[(E3-independent) ubiquitin-conjugating enzyme]-L-cysteine
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-156441 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Ubiquitin-conjugating enzyme E2 E1 Chain: A
Molecule details ›
Chain: A
Length: 194 amino acids
Theoretical weight: 21.49 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P51965 (Residues: 1-193; Coverage: 100%)
Gene names: UBCH6, UBE2E1
Sequence domains: Ubiquitin-conjugating enzyme
Structure domains: Ubiquitin Conjugating Enzyme

Ligands and Environments

1 bound ligand:
Ligand GOL 1 x GOL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 23-ID-B
Spacegroup: P3121
Unit cell:
a: 51.63Å b: 51.63Å c: 109.437Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.206 0.205 0.226
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

12 review citations

E2 enzymes: more than just middle men.
Stewart et al. (2016)
11 more

7 mentions without citation

A human ubiquitin conjugating enzyme (E2)-HECT E3 ligase structure-function screen.
Sheng et al. (2012)
6 more