› 3c0z

X-ray diffraction
2.1Å resolution

Crystal structure of catalytic domain of human histone deacetylase HDAC7 in complex with SAHA

Released:
DOI: 10.2210/pdb3c0z/pdb
PDB entry 3c0z coloured by chain, front view.
PDB entry 3c0z coloured by chain, side view.
PDB entry 3c0z coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Human HDAC7 harbors a class IIa histone deacetylase-specific zinc binding motif and cryptic deacetylase activity.
Schuetz A, Min J, Allali-Hassani A, Schapira M, Shuen M, Loppnau P, Mazitschek R, Kwiatkowski NP, Lewis TA, Maglathin RL, McLean TH, Bochkarev A, Plotnikov AN, Vedadi M, Arrowsmith CH
J Biol Chem 283 11355-63 (2008)
PMID: 18285338

Function and Biology Details

Reaction catalysed: 
Hydrolysis of an N(6)-acetyl-lysine residue of a histone to yield a deacetylated histone
Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-186716 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Histone deacetylase 7 Chains: A, B, C
Molecule details ›
Chains: A, B, C
Length: 423 amino acids
Theoretical weight: 45.52 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q8WUI4 (Residues: 482-903; Coverage: 44%)
Gene names: HDAC7, HDAC7A
Sequence domains: Histone deacetylase domain
Structure domains: Histone deacetylase domain

Ligands and Environments

3 bound ligands:
Ligand ZN 6 x ZN
Ligand K 6 x K
Ligand SHH 3 x SHH
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X25
Spacegroup: P32
Unit cell:
a: 81.825Å b: 81.825Å c: 148.867Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.199 0.197 0.243
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

58 review citations

The many roles of histone deacetylases in development and physiology: implications for disease and therapy.
Haberland et al. (2009)
57 more

23 mentions without citation

Modulation of epigenetic targets for anticancer therapy: clinicopathological relevance, structural data and drug discovery perspectives.
Andreoli et al. (2013)
22 more