› 3c3t

X-ray diffraction
2.2Å resolution

Role of a Glutamate Bridge Spanning the Dimeric Interface of Human Manganese Superoxide Dismutase

Released:
DOI: 10.2210/pdb3c3t/pdb
PDB entry 3c3t coloured by chain, front view.
PDB entry 3c3t coloured by chain, side view.
PDB entry 3c3t coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Role of a glutamate bridge spanning the dimeric interface of human manganese superoxide dismutase.
Quint PS, Domsic JF, Cabelli DE, McKenna R, Silverman DN
Biochemistry 47 4621-8 (2008)
PMID: 18373354

Function and Biology Details

Reaction catalysed: 
2 superoxide + 2 H(+) = O(2) + H(2)O(2)
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:
Structure domains:

Structure analysis Details

Assemblies composition:
homo tetramer
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-137484 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Superoxide dismutase [Mn], mitochondrial Chains: A, B
Molecule details ›
Chains: A, B
Length: 198 amino acids
Theoretical weight: 22.22 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P04179 (Residues: 25-222; Coverage: 89%)
Gene name: SOD2
Sequence domains:
Structure domains:

Ligands and Environments

2 bound ligands:
Ligand MN 2 x MN
Ligand SO4 2 x SO4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU RUH3R
Spacegroup: P6122
Unit cell:
a: 81.293Å b: 81.293Å c: 241.693Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.175 0.175 0.201
Expression system: Escherichia coli

Quick links

Citations

1 review citation

A Review of the Catalytic Mechanism of Human Manganese Superoxide Dismutase.
Azadmanesh et al. (2018)
7 other articles

1 mention without citation

The structure-function relationships and physiological roles of MnSOD mutants.
Bonetta Valentino R. (2022)