Function and Biology Details
Reactions catalysed:
[a protein]-serine/threonine phosphate + H(2)O = [a protein]-serine/threonine + phosphate
[Phosphatase 2A protein]-leucine methyl ester + H(2)O = [phosphatase 2A protein]-leucine + methanol
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
- Serine/threonine-specific protein phosphatase/bis(5-nucleosyl)-tetraphosphatase
- HEAT, type 2
- Alpha/Beta hydrolase fold
- Protein phosphatase methylesterase, eukaryotic
- Metallo-dependent phosphatase-like
- Armadillo-like helical
- Alpha/beta hydrolase fold-1
- Calcineurin-like phosphoesterase domain, ApaH type
3 more domains
Structure domain:
Structure analysis Details
Assembly composition:
hetero trimer (preferred)
PDBe Complex ID:
PDB-CPX-151668 (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
Serine/threonine-protein phosphatase 2A 65 kDa regulatory subunit A alpha isoform
Molecule details ›
Chain: A
Length: 232 amino acids
Theoretical weight: 25.9 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
Sequence domains: HEAT repeat
Structure domains: Leucine-rich Repeat Variant
Length: 232 amino acids
Theoretical weight: 25.9 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
- Canonical:
P30153 (Residues: 364-589; Coverage: 39%)
Sequence domains: HEAT repeat
Structure domains: Leucine-rich Repeat Variant
Serine/threonine-protein phosphatase 2A catalytic subunit alpha isoform
Molecule details ›
Chain: C
Length: 310 amino acids
Theoretical weight: 35.69 KDa
Source organism: Homo sapiens
Expression system: Spodoptera frugiperda
UniProt:
Sequence domains: Calcineurin-like phosphoesterase
Structure domains: Purple Acid Phosphatase; chain A, domain 2
Length: 310 amino acids
Theoretical weight: 35.69 KDa
Source organism: Homo sapiens
Expression system: Spodoptera frugiperda
UniProt:
- Canonical: P67775 (Residues: 1-309; Coverage: 100%)
Sequence domains: Calcineurin-like phosphoesterase
Structure domains: Purple Acid Phosphatase; chain A, domain 2
Protein phosphatase methylesterase 1
Molecule details ›
Chain: P
Length: 310 amino acids
Theoretical weight: 34.09 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
Sequence domains: Alpha/beta hydrolase family
Structure domains: alpha/beta hydrolase
Length: 310 amino acids
Theoretical weight: 34.09 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
- Canonical: Q9Y570 (Residues: 39-386; Coverage: 79%)
Sequence domains: Alpha/beta hydrolase family
Structure domains: alpha/beta hydrolase
Ligands and Environments
No bound ligands
No modified residues
Experiments and Validation Details
X-ray source:
NSLS BEAMLINE X29A
Spacegroup:
C2
Expression systems:
- Escherichia coli
- Spodoptera frugiperda
