3c60

X-ray diffraction
3.05Å resolution

Crystal structure of mouse MHC class II I-Ab/3K peptide complexed with mouse TCR YAe62

Released:

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
PDBe Complex ID:
PDB-CPX-210597 (preferred)
Entry contents:
4 distinct polypeptide molecules
Macromolecules (4 distinct):
TCR YAe62 alpha chain Chains: A, E
Molecule details ›
Chains: A, E
Length: 199 amino acids
Theoretical weight: 22.11 KDa
Source organism: Mus musculus
Expression system: Escherichia coli BL21
Structure domains: Immunoglobulins
TCR YAe62 beta chain Chains: B, F
Molecule details ›
Chains: B, F
Length: 236 amino acids
Theoretical weight: 26.47 KDa
Source organism: Mus musculus
Expression system: Escherichia coli BL21
Structure domains: Immunoglobulins
H-2 class II histocompatibility antigen, A-B alpha chain Chains: C, G
Molecule details ›
Chains: C, G
Length: 182 amino acids
Theoretical weight: 20.6 KDa
Source organism: Mus musculus
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: P14434 (Residues: 27-208; Coverage: 78%)
Gene name: H2-Aa
Sequence domains:
Structure domains:
H-2 class II histocompatibility antigen, A beta chain Chains: D, H
Molecule details ›
Chains: D, H
Length: 217 amino acids
Theoretical weight: 24.94 KDa
Source organism: Mus musculus
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: P14483 (Residues: 21-218; Coverage: 77%)
Gene names: H2-Ab1, H2-iabeta
Sequence domains:
Structure domains:

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 19-ID
Spacegroup: P212121
Unit cell:
a: 66.058Å b: 126.168Å c: 277.184Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.267 0.267 0.309
Expression systems:
  • Escherichia coli BL21
  • Spodoptera frugiperda