› 3cb5

X-ray diffraction
2.05Å resolution

Crystal Structure of the S. pombe Peptidase Homology Domain of FACT complex subunit Spt16 (form A)

Released:
DOI: 10.2210/pdb3cb5/pdb
PDB entry 3cb5 coloured by chain, front view.
PDB entry 3cb5 coloured by chain, side view.
PDB entry 3cb5 coloured by chain, top view.
Source organism: Schizosaccharomyces pombe
Primary publication:
The FACT Spt16 "peptidase" domain is a histone H3-H4 binding module.
Stuwe T, Hothorn M, Lejeune E, Rybin V, Bortfeld M, Scheffzek K, Ladurner AG
Proc Natl Acad Sci U S A 105 8884-9 (2008)
PMID: 18579787

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-131706 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
FACT complex subunit spt16 Chains: A, B
Molecule details ›
Chains: A, B
Length: 444 amino acids
Theoretical weight: 50.22 KDa
Source organism: Schizosaccharomyces pombe
Expression system: Escherichia coli
UniProt:
  • Canonical: O94267 (Residues: 1-442; Coverage: 43%)
Gene names: SPBP8B7.19, spt16
Sequence domains:
Structure domains:

Ligands and Environments

1 bound ligand:
Ligand B3P 1 x B3P
1 modified residue:
Ligand MSE 10 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE BM16, SLS BEAMLINE X06SA
Spacegroup: P212121
Unit cell:
a: 97.07Å b: 101.65Å c: 103.51Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.208 0.206 0.245
Expression system: Escherichia coli

Quick links

Citations

18 review citations

Histone chaperones in nucleosome assembly and human disease.
Burgess et al. (2013)
17 more

6 mentions without citation

Chaperoning histones during DNA replication and repair.
Ransom et al. (2010)
5 more