› 3cb6

X-ray diffraction
1.84Å resolution

Crystal Structure of the S. pombe Peptidase Homology Domain of FACT complex subunit Spt16 (form B)

Released:
DOI: 10.2210/pdb3cb6/pdb
PDB entry 3cb6 coloured by chain, front view.
PDB entry 3cb6 coloured by chain, side view.
PDB entry 3cb6 coloured by chain, top view.
Source organism: Schizosaccharomyces pombe
Primary publication:
The FACT Spt16 "peptidase" domain is a histone H3-H4 binding module.
Stuwe T, Hothorn M, Lejeune E, Rybin V, Bortfeld M, Scheffzek K, Ladurner AG
Proc Natl Acad Sci U S A 105 8884-9 (2008)
PMID: 18579787

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
Sequence domains:

Structure analysis Details

Assemblies composition:
monomeric (preferred)
homo dimer
Assembly name:
PDBe Complex ID:
PDB-CPX-131706 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
FACT complex subunit spt16 Chain: A
Molecule details ›
Chain: A
Length: 444 amino acids
Theoretical weight: 49.99 KDa
Source organism: Schizosaccharomyces pombe
Expression system: Escherichia coli
UniProt:
  • Canonical: O94267 (Residues: 1-442; Coverage: 43%)
Gene names: SPBP8B7.19, spt16
Sequence domains:
Structure domains:

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: DIAMOND BEAMLINE I04
Spacegroup: C2221
Unit cell:
a: 62.98Å b: 193.67Å c: 89.48Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.193 0.192 0.225
Expression system: Escherichia coli

Quick links

Citations

22 review citations

Chaperoning histones during DNA replication and repair.
Ransom et al. (2010)
21 more

2 mentions without citation

Structure-function relationship of H2A-H2B specific plant histone chaperones.
Kumar et al. (2020)
1 more